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KMID : 1134820120410091205
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Journal of the Korean Society of Food Science and Nutrition
2012 Volume.41 No. 9 p.1205 ~ p.1210
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Anti-Inflammatory Effects of Extracts from Ligustrum ovalifolium H. Leaves on RAW264.7 Macrophages
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Kim Yon-Suk
Lee Seung-Jae
Hwang Jin-Woo
Kim Ee-Hwa
Park Pyo-Jam
Jeong Jae-Hyun
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Abstract
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This study investigated the anti-inflammatory effects of Ligustrum ovalifolium H. (LOH) leaf extracts on RAW264.7 macrophages. Cell toxicity was determined by MTT assay. We evaluated the anti-inflammatory effects of LOH extracts by measuring nitric oxide (NO), reactive oxygen species (ROS), inducible NOS (iNOS) production, and cyclooxygenase-2 (COX-2) expression by Western blotting. LOH ethanolic extracts (0.05, 0.1, and 0.2 mg/mL) significantly suppressed LPS-stimulated production of NO. The intracellular ROS level also significantly decreased. LOH ethanolic extracts reduced the expression of iNOS and COX-2 proteins. The present results show that LOH ethanol extract has potent anti-inflammatory effects on RAW264.7 macrophages. These results also suggest that the anti-inflammatory effects of LOH extracts may be related to the inhibition of LPS-stimulated ROS and NO production. Therefore, ethanolic extracts of LOH leaves may be utilized as a good source of functional foods for protection against inflammatory diseases.
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KEYWORD
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anti-inflammation, Ligustrum ovalifolium H., nitric oxide, nitric oxide synthase, reactive oxygen species (ROS)
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